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Sunday, July 9, 2017

Introduction to Biochemistry - Quiz 2.4.4

I got all the answers correct this time all on the first attempt!


Notice Vmax doesn't change - this is a characteristic for competitive inhibition. Vmax does not change because the inhibition is done by competing with enzyme with the substrate, once the substrate concentration increase the inhibition will eventually give way to the substrate and therefore Vmax stay constant.


This is Noncompetitive - it is confusing because we have uncompetitive and noncompetitive. Uncompetitive means the inhibitor does not compete with the substrate for the enzyme, instead, it binds to the substrate enzyme complex to inhibit the reaction by not allowing the enzyme substrate complex to turn into product. In that case the lines would be parallel. Noncompetitive is confusing because it is actually competiting, but it is competing and also blocking the enzyme substrate complex to turn into product, to the point that km is identical with the enzyme not inhibited.

Strange name - I have to say, a non-competitive inhibition is actually using the competitive mechanism.


A noncompetitive inhibitor is a special case, where apparent km is the same as for the enzyme without the inhibitor, while the Vmaxisdecreased.Inthemathematicalexpressionabove,thevalueof \alpha isequaltothevalueof \alpha '$


Remember for competitive inhibitor the line have the same y intercept and bigger slope, therefore 1km is smaller, therefore km is larger.

It increase the apparent km of the enzyme
It binds the enzyme complex in its active site.


This is similar to the above, because the line is parallel, uncompetitive inhibitor decreases km and vmax

It decreases the apparent km of the enzyme.
It binds the enzyme-substrate complex.


The above equation represents the Lineweaver-Burk equation for an enzyme with a mixed inhibitor.

This is so because we have two alphas.


Note that the slope of the equation does not change, it is the parallel line case.

The above equation represents the Lineweaver-Burk equation for an enzyme with an uncompetitive inhibitor.



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